关于THFC库存

现存有超过8000株转基因RNAi品系,约200株Gal4品系,详情查阅附表。RNAi constructs经以下4个载体改造而来:VALIUM1, VALIUM10, VALIUM20 and VALIUM22。如下表所示
Vector name Integrate site Discribe Reference
VALIUM1 attp2,on 3rd chromosome works in soma;VALIUM1 is the "weakest" and very likely require the inclusion of UAS-Dicer2 for maximum effect Ni et al., 2008, Nature Methods
VALIUM10 attp2,on 3rd chromosome works well in soma but usually not as well as VALIUM20 Ni et al., 2009, genetics.
VALIUM20 attp2,on 3rd chromosome,or attp40,on 2nd chromosome works well in both soma and germline Ni et al., 2011, Nature Methods
VALIUM22 attp2,on 3rd chromosome,or attp40,on 2nd chromosome works significantly in female germline; do not work well in soma Ni et al., 2011, Nature Methods

订购指南

  1. 从网站搜索下载果蝇库列表并找到您感兴趣的果蝇品系。
  2. 填写订购单
  3. 将表格发送给果蝇中心助理乔欢欢(qiaohh0214@163.com)和中心负责人刘老师(lpliu@mail.tsinghua.edu.cn)以确认订单。
  4. 首次订购需要在材料转移协议(MTA)中签名并加盖公章后将扫描件通过邮件发给我们。如需订购nos-Cas9转基因果蝇,请签订fly-center MTA
  5. 有意下单者请填写果蝇订购表,并以订购单位+PI姓名+订购日期(年-月-日)来命名邮件主题,谢谢配合。

您所订购的果蝇订单需要约三天时间处理,届时您将被邮件通知自取或发货(北京地区订单一律自取)。

对于其他城市的顾客,您订购的果蝇将用EMS发出,运费自理。

价格

我们的果蝇订单的费用(不包含运费)以及订单折扣跟Bloomington果蝇中心相仿。单次的订单也享受折扣,因此不必一次订全。

Stocks Ordered Cost per Stock (¥)
1-5 200(around 32 USD)
6-100 100(around 14 USD)
>100 40(around 6 USD)
e.g: if you order 150 lines in 2013
5×200=1000
95×100=9500
50×40=2000
Then you should pay: 1000+9500+2000=12500
Acknowledge THFC
Please acknowledge the TsingHua Fly Center in your related publications and cite Ni et al., 2008, 2011, Nature Methods. It is also important to specify the TRiP# (Short Hairpin) number in your Materials and Methods section so that your research is reproducible.