About THFC StocksMore than 8000 RNAi lines, and about 200 Gal4 lines are available in THFC, see list details. The RNAi constructs are based on four different vectors -- VALIUM1, VALIUM10, VALIUM20 and VALIUM22, as follows
|Vector name||Integrate site||Discribe||Reference|
|VALIUM1||attp2,on 3rd chromosome||works in soma;VALIUM1 is the "weakest" and very likely require the inclusion of UAS-Dicer2 for maximum effect||Ni et al., 2008, Nature Methods|
|VALIUM10||attp2,on 3rd chromosome||works well in soma but usually not as well as VALIUM20||Ni et al., 2009, genetics.|
|VALIUM20||attp2,on 3rd chromosome,or attp40,on 2nd chromosome||works well in both soma and germline||Ni et al., 2011, Nature Methods|
|VALIUM22||attp2,on 3rd chromosome,or attp40,on 2nd chromosome||works significantly in female germline; do not work well in soma||Ni et al., 2011, Nature Methods|
How to order
- Search or download the stock list from the website and find the lines you are interested in.
- Fill in the order form.
- Send the form to Huanhuan Qiao (firstname.lastname@example.org) and Luping Liu(email@example.com) to make clear your purpose.
- If it’s the first time you order lines form THFC, there’s an additional step: sign and stamp official seal on the Material Transfer Agreement (*must be signed by the supervisor), then fax the scanned document to us. For orders of our nos-Cas9 transgenic flies, you need to sign fly-center MTA.
All purchased stocks will be confirmed by us to have fresh larvae (about three days) before pick up or shipping out.
For customers in Beijing, you can pick up the flies yourselves. For customers in other cities, the flies will be shipped via EMS by freight collect.
The stock fee (not including shipping fee) and discount are similar to those of the Bloomington stock center. The number of stocks ordered is cumulative through the calendar year and need not be ordered all at once to obtain the discounted price. For each ordering, please fill a blank Stock Order Form. The subject of order email must be as school＋supervisor＋date.
|Stocks Ordered||Cost per Stock (￥)|
|1-5||200(around 32 USD)|
|6-100||100(around 14 USD)|
|>100||40(around 6 USD)|
|e.g: if you order 150 lines in 2013
Then you should pay: 1000+9500+2000=12500