About THFC Stocks

More than 8000 RNAi lines, and about 200 Gal4 lines are available in THFC, see list details. The RNAi constructs are based on four different vectors -- VALIUM1, VALIUM10, VALIUM20 and VALIUM22, as follows
Vector name Integrate site Discribe Reference
VALIUM1 attp2,on 3rd chromosome works in soma;VALIUM1 is the "weakest" and very likely require the inclusion of UAS-Dicer2 for maximum effect Ni et al., 2008, Nature Methods
VALIUM10 attp2,on 3rd chromosome works well in soma but usually not as well as VALIUM20 Ni et al., 2009, genetics.
VALIUM20 attp2,on 3rd chromosome,or attp40,on 2nd chromosome works well in both soma and germline Ni et al., 2011, Nature Methods
VALIUM22 attp2,on 3rd chromosome,or attp40,on 2nd chromosome works significantly in female germline; do not work well in soma Ni et al., 2011, Nature Methods

How to order

  1. Search or download the stock list from the website and find the lines you are interested in.
  2. Fill in the order form.
  3. Send the form to Huanhuan Qiao (qiaohh0214@163.com) and Luping Liu(lpliu@mail.tsinghua.edu.cn) to make clear your purpose.
  4. If it’s the first time you order lines form THFC, there’s an additional step: sign and stamp official seal on the Material Transfer Agreement (*must be signed by the supervisor), then fax the scanned document to us. For orders of our nos-Cas9 transgenic flies, you need to sign fly-center MTA.

All purchased stocks will be confirmed by us to have fresh larvae (about three days) before pick up or shipping out.

For customers in Beijing, you can pick up the flies yourselves. For customers in other cities, the flies will be shipped via EMS by freight collect.

Cost

The stock fee (not including shipping fee) and discount are similar to those of the Bloomington stock center. The number of stocks ordered is cumulative through the calendar year and need not be ordered all at once to obtain the discounted price. For each ordering, please fill a blank Stock Order Form. The subject of order email must be as school+supervisor+date.

Stocks Ordered Cost per Stock (¥)
1-5 100(around 16 USD)
6-100 50(around 7 USD)
>100 20(around 3 USD)
e.g: if you order 150 lines in 2013
5×100=500
95×50=4750
50×20=1000
Then you should pay: 500+4750+1000=6250
Acknowledge THFC
Please acknowledge the TsingHua Fly Center in your related publications and cite Ni et al., 2008, 2011, Nature Methods. It is also important to specify the TRiP# (Short Hairpin) number in your Materials and Methods section so that your research is reproducible.